Conjugation of 2,6-Dichloroindophenol with Mitochondrial Thiol Groups

Conjugation of 2,6-dichloroindophenol with mitochondrial thiol groups.

Substantial quantities of 2,6-dichloroindophenol are bound to mitochondrial constituents. It is likely that binding is mediated by conjugation of dye to thiol groups, since reaction conditions favoring binding are completely analogous to conditions favorable for the conjugation of dye to isolated thiol compounds. Thus, procedures which promote the maintenance of reduced 2,6-dichloroindophenol (...

Gold Solid Surface Thiol Oligo Conjugation

The thiol groups of fumarase.

Several reagents have been employed to determine the role of the thiol groups in fumarase. The nature of the reaction of these reagents indicates that the thiol groups are not associated with active site structures but are buried in a hydrophobic environment in the interior of the enzyme. Several observations are in accord with this view. The rate of reaction of the thiol groups is much slower ...

Pyrrolobenzodiazepine Antibody-Drug Conjugates Designed for Stable Thiol Conjugation

Thiosuccinimide-linked antibody-drug conjugates (ADCs) are susceptible to drug loss over time due to a retro-Michael reaction, which can be prevented by selecting stable conjugation positions or hydrolysis of the thiosuccinimide. Here, we investigate pyrrolobenzodiazepine (PBD) ADC drug-linkers equipped with N-phenyl maleimide functionality for stable thiol conjugation via thiosuccinimide hydro...

A new reagent for stable thiol-specific conjugation.

Many clinically used protein therapeutics are modified to increase their efficacy. Example modifications include the conjugation of cytotoxic drugs to monoclonal antibodies or poly(ethylene glycol) (PEG) to proteins and peptides. Monothiol-specific conjugation can be efficient and is often accomplished using maleimide-based reagents. However, maleimide derived conjugates are known to be suscept...